On the mid-Atlantic coast between 1624 and 1664, the Dutch developed a successful and expansive colony, one that depended on particular interactions among women and men from American, European, and African backgrounds. Unlike some other colonial efforts, such as Jamestown, New Netherland had white women colonists from its inception. In contrast to Plymouth and other English settler colonies, a population of African men and women did the crucial work of establishing the colony’s initial infrastructure in its first years. What is more, a thriving cross-cultural trade between Netherlanders and Munsee, Mahican, and Mohawk residents of the region nurtured the development of the infant colony. Looking at the colony’s establishment and growth reveals that complex interactions among ethnically distinct families gave New Netherland its particular form and character. As European and African populations took root, many households engaged in the frontier trading economy, creating a web of connections reaching into multiple indigenous villages. Women and men cooperated to sustain this trade over long distances by relying on marriage and the economic unit of the household to organize production and exchange. In addition, the colonial government used these households to stake claims to the ground and to define Dutch jurisdiction, just as they recognized that residence by Indian or English households determined where Dutch power ended. Thus ethnic and gender relations shaped not only the colony’s internal hierarchies, but also its economy and its very boundaries.