Modern zoos emerged as mass entertainment, spaces of public leisure and of culture. In the past, they served as monuments and expressions of the degree of “civilization” and progress of a city and its respective country. In Latin America, zoos date from the last quarter of the 19th century. The history of Latin American zoos is a political, cultural, and social history. The conditions of their creation and operation over the decades have conferred important specificities to these institutions. Since their inception, zoos in Latin America have reflected nationalistic aspirations, civilizational projects, and social transformation. Over the decades, the history of many zoos has blended with natural history in Latin America, as many zoo founders were important scientists. The development of new sensitivities toward animals also follows the history of zoos in Latin America from the beginning, because the first animal protection societies appeared at the same time. Today, zoos face vigorous claims from animal rights activists calling for their closure. In view of so many challenges, these institutions are reinventing themselves with an increased focus on conservation and environmental education, joining international zoological societies with high standards of quality. Among several of these societies, the Latin American Association of Zoological Parks and Aquariums (ALPZA) stands out. Founded in 1990, ALPZA organizes, reshapes, and integrates Latin American zoos, establishing global connections. Various actors play a role in the defense and contestation of zoos, such as politicians, scientists, conservationists, animal protection societies, anti-zoo activists, visitors, administrators, officials, and, of course, thousands of wild animals from all over the world who have lived in Latin American cities for decades.