Latinx literature’s historical interest in the cultural, social, and political dynamics of gender plays as central a role in its long and varied discursive tradition as any other major thematic concern. Since the 19th century, representations of life in Latinx communities inhabiting what increasingly became the territory of the United States put the forces and conflicts of culturally based gender differences center stage, whether those differences came from within a culture, whose values shifted when it moved to a new geographic setting, or from without, when a culture confronted the differing values of an often dominant, oppressive other. Latinx literature is too vast and varied to accommodate a comprehensive account of these shifts and currents. But one can see a steady move away from the rigid binary logic of gender difference inherited from the traditional cis-hetero-patriarchal mindset of colonial Spanish-Catholic rule, a mindset that, historically, overwhelmed whatever more fluid or ambiguous formations of gender and sexuality circulated through indigenous American societies. That steady move cannot be traced in a single line or direction, but it does clearly demonstrate a greater opening of the possibility of dislodging gendered styles of expression from the particular anatomical manifestations of sexed bodies, as well as a greater opening of the possibility for mixed lines of attraction and desire between, within, and even beyond genders. While much liberatory work remains to be done in the actual world, Latinx literature has increasingly opened itself up to more inclusive, affirmative representations of nonnormative lives under the signs of sexuality and gender.