Figure 3. The long-term memory (LTM) protocol involves trained worms receiving spaced training, where 4 blocks of 20 taps are delivered at a 60-s ISI with 1 rest period between each training block, while control worms receive 1 tap. After 24 h, 10 taps at a 60-s ISI are delivered to both control and trained worms to test for memory. The intermediate-term memory (ITM) protocol involves trained worms receiving massed training with 80 taps at a 60-s ISI, while control worms receive 1 tap. After 12 h, 10 taps at a 60-s ISI are delivered to both control and trained worms to test for memory.