Figure 7. On the left, a comparison of the amphioxus neurotransmitters map (from 20–24 hr old embryos) with TEM data (from 12,5 days old larvae). Commissural neurons (CN), third pair of large paired neurons (LPN3); motoneurons (MN), multipolar neurons (MP), ipsilateral projection neurons (IPN). On the right, ISH image from 24 hr embryo showing VAChT (dark purple) and VGLUT (yellow) expression, markers for cholinergic and glutamatergic neurons, respectively (redrawn from Lacalli & Candiani, 2017).